Arterial hypertension as a predictor of morpho- densitometric changes development in rats` solitary-vagal complex

M. V. Danukalo, O. V. Melnikova

Abstract


The components of the dorsal vagal complex: the nucleus of the solitary tract (NST) and the dorsal motor nucleus (DMN) are key structures in BP regulation. The maintenance of BP normal level depends on functional state of these structures. it is possible to estimate the neuron morpho-functional state not only with direct electrophysiological methods but also analyzing the nucleus size and nucleic acid (NA) concentration in it. Therefore, the aim of our work was to determine the features of morpho-densitometric indices in the nuclei of the NST and DMN neurons in AH of various origins in experimental animals and to give a comparative analysis of these data. Materials and methods: the study was carried out on 30 mature male rats: 10 control Wistar rats, 10 Wistar rats with modeled endocrine-salt AH (ESAH) and 10 spontaneously hypertensive rats (SHR) which are well-established model of human essential arterial hypertension (EAH). The histochemical method of staining with gallocyanine-chrome alum by Einarson was used for the evaluation of morpho-densitometric characteristics of the DMN and NTS neurons` nuclei: the nucleus area, the content and concentration of nucleic acids in the nucleus.
Conclusions: The following conclusions can be made as a research result: 1. Morpho-densitometric changes occur in NTS and DMN in arterial hypertension, regardless of its pathogenesis.
2. The most distinct changes of the studied indices of neurons` nuclei were observed in DMN of the rats with EAH. Although only the neurons` nuclei area significantly changed in the NTS. The distribution of neurons` nuclei by their area in DMN and NTS in SHR rats showed a downward trend.
3. Significant decrease in all morpho-densitometric characteristics of the neurons` nuclei is observed in the DMN in the rats with an ESAH. The distribution of neurons` nuclei by their area showed a tendency to decrease. In the nuclei of NST neurons, only the content and concentration of NA were reliably low in comparison with control animals. The distribution curve of neurons by the area of their nuclei in ESAH rats is characterized by wider variability of size than in the control group.

Keywords


nucleus of the solitary tract, dorsal motor nucleus, arterial hypertension, rats

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References


Marina, N., Ang, R., Machhada, A., Kasymov, V., Karagiannis, A., Hosford, P. S & Kasparov, S. (2015). Brainstem hypoxia contributes to the development of hypertension in the spontaneously hypertensive rat. Hypertension, 65(4), 775-783.

Kolpakova, J., Li, L., Hatcher, J. T., Gu, H., Zhang, X., Chen, J., & Cheng, Z. J. (2017). Responses of Nucleus Tractus Solitarius (NTS) early and late neurons to blood pressure changes in anesthetized F344 rats. PloS one, 12(4), e0169529.

Hosford, P. S., Millar, J., Ramage, A. G., & Marina, N. (2017). Abnormal oxygen homeostasis in the nucleus tractus solitarii of the spontaneously hypertensive rat. Experimental physiology, 102(4), 389-396.

Gregory, M. D., & Benarroch, E. E. (2017). Nucleus of the solitary tract, medullary reflexes, and clinical implications.

Sabbatini, M., Grossini, E., Molinari, C., Mary, D. A., Vacca, G., & Cannas, M. (2017). Gastric distension causes changes in heart rate and arterial blood pressure by affecting the crosstalk between vagal and splanchnic systems in anesthetised rats. Experimental brain research, 235(4), 1081-1095.

Gourine, A. V., Machhada, A., Trapp, S., & Spyer, K. M. (2016). Cardiac vagal preganglionic neurones: An update. Autonomic Neuroscience, 199, 24-28.

Cheng, Z., Zhang, H., Guo, S. Z., Wurster, R., & Gozal, D. (2004). Differential control over postganglionic neurons in rat cardiac ganglia by NA and DmnX neurons: anatomical evidence. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology, 286(4), R625-R633.

Blanch, G. T., Freiria-Oliveira, A. H., Speretta, G. F. F., Carrera, E. J., Li, H., Speth, R. C., ... & Colombari, D. S. (2014). Increased expression of angiotensin II type 2 receptors in the solitary–vagal complex blunts renovascular hypertension. Hypertension, 64(4), 777-783.

Dornas, W. C., & Silva, M. E. (2011). Animal models for the study of arterial hypertension. Journal of biosciences, 36(4), 731-737.

Kolesnyk Y, Gancheva O, Abramov A, Ivanenko T, Tischenko S, Kuzo N, inventors; Zaporizhzhya State Medical University, assignee. Sposib modelyuvannya symptomatychnoy arterial`noi hypertezii u dribnyh gryzuniv [Method of simulation of symptomatic arterial hypertension in small rodents]. Ukraine patent 102234, 2015 Oct. 26. (Ukrainian)

Kolesnyk, Yu. M., Hancheva, O. V., Abramov, A. V., Kolesnyk, M. Yu., Ivanenko, T. V., Tishсhenko, S. V., et al. (2017). Sovremennye podkhody i novye metodicheskie vozmozhnosti v ocenke funkcional’nogo sostoyaniya melkikh laboratornykh zhivotnykh [Modern approaches and new methodological possibilities in the functional state of small laboratory animals assessing]. Pathologia, 3(41), 364–370. doi: 10.14739/2310- 1237.2017.3.118770 [in Russian]

Paxinos G, Watson C. The Rat in Stereotaxic Coordinates. New York, Academic Press; 1986.

Pearse, A. G. E. (1962). Gistokhimiya. Teoreticheskaya i prikladnaya [Histochemistry, Theoretical and Applied]. Moscow: Izdatel`stvo inostrannoy literatyri. [in Russian].

Ferreira, T., & Rasband, W. (2012). ImageJ user guide. ImageJ/Fiji, 1, 155-161.

Zaycev V, Liflyandskiy V. Prikladnaya meditsynskaya statistika. Uchebno-practicheskoye posobiye. [Applied medical statistics. Teaching aid] 1st ed. Sankt-Peterburg: Foliant; 2003. [in Russian]

Lafarga, M., & Berciano, M. T. (1985). A Golgi and morphometric study of the ectopic granule cells in the molecular layer of the rat cerebellum. Brain research, 345(2), 398-401.

Nelson, D. O., & Boulant, J. A. (1983). Altered brainstem structure of spontaneously hypertensive (SHR) rats. Brain research, 261(1), 145-150.

Basting, T., & Lazartigues, E. (2017). DOCA-salt hypertension: an update. Current hypertension reports, 19(4), 32.

McMenamin, C. A., Anselmi, L., Travagli, R. A., & Browning, K. N. (2016). Developmental regulation of inhibitory synaptic currents in the dorsal motor nucleus of the vagus in the rat. Journal of neurophysiology, 116(4), 1705-1714.

Melo, M. R., Gasparini, S., Speretta, G. F., Silva, E. F., Pedrino, G. R., Menani, J. V., Colombari, E. (2019). Importance of the commissural nucleus of the solitary tract in renovascular hypertension. Hypertension Research, 42(5), 587.

Tsai, H. W., & Davenport, P. W. (2016). Activation of Neurons in the Intermediate and Medial Nucleus of Tractus Solitarius by Multiple Afferent Stimuli. The FASEB Journal, 30(1_supplement), 1261-6.




DOI: http://dx.doi.org/10.5281/zenodo.3497436

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