The study of leptin concentration in saliva during orthodontic treatment, periodontal diseases and wound healing in the oral mucosa-review

Izabela Cwalina, Marta Komarowska, Ewelina Dargiewicz, Adam Hermanowicz


The oral cavity is a very complex environment characterized by numerous interactions between different tissues, secretions from various glands, foods, air and microorganisms. Leptin is a hormone-like protein, which plays an important role in protection of host against inflammation and infection. It exhibits many physiological properties. Some studies have reported changes in leptin levels during orthodontic treatment, wound healing, periodontal disease. The aim of the paper was to review the salivary leptin role in each of these cases.
Articles published before November 2018 were obtained from PubMed, Dentistry & Oral Sciences Source. Inclusion criteria were: a) empirically based b) peer-reviewed.
Discussion and results
Salivary leptin levels significantly decrease during orthodontic tooth movement and periodontal diseases. However, it increases while wound healing in the oral mucosa.
There is a correlation between tooth movement and leptin level. Obese patients could have lesser tooth movement. This hormone promotes wound healing and angiogenesis in oral cavity. Leptin concentration in saliva increases in patients with periodontitis.


saliva; leptin; healing; orthodontic tooth movement; periodontitis

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Buczko P, Knaś M, Grycz M, Szarmach I, Zalewska A. Orthodontic treatment modifies the oxidant-antioxidant balance in saliva of clinically healthy subjects. Adv in Med Sci 2017;62;129-135.

Thanakun S, Watanabe H, Thaweboon S, Izumi, Y. An effective techique for the processing of saliva for the analysis of leptin and adiponectin. Peptides 2013;47;60-65.

Mamali I, Roupas N, Armeni A, Theodoropoulou A, Markou K, Georgopoulos N. Measurment of salivary resistin, visfatin and adiponectin levels. Peptides 2012;33;120-124.

Toda M, Tsukinoki R, Morimoto K. Measurment of salivary adiponectin levels. Acta Diabetol 2007;44;20-22.

Loo J, Yan W, Ramachan P, Wong D. Comparative human salivary and plasma proteomes. J Dent Res 2010;89;1016-1023.

Pfaffe T, Cooper-White J, Beyerlein P, Kostner K, Punyadeera C. Diagnostic potential of saliva: current state and future applications. Clin Chem 2011;57;675-687.

Jiang J, Park N, Hu S, Wong D. A universal pre-analytic solution for concurrent stabilization of salivary proteins, RNA and DNA at ambient temperaturę. Arch Oral Biol 2009;54;268-273.

Jayachandran T, Srinivasan B, Padmanabhan S. Salivary leptin levels in normal weight and overweight individuals and their correlation with orthodontic tooth movement. Angle Orthod. 2017; 87(5);739-744.

Reseland J, Syversen U, Bakke I, et al. Leptin is expressed in and secreted from primary cultures of human osteoblasts and promotes bone mineralization. J Bone Miner Res. 2001;16;1426-1433.

Groschl M, Rauh M, Wagner R et al. Identification of leptin in human saliva. J Clin Endocrinol Metab. 2001;86;5234-5239.

Randeva H, Karteris E, Lewandowski K, Sailesh S, O’Hare P, Hillhouse E. Circadian rhythmicity of salivary leptin in healthy subjects. Mol Genet Metab. 2003;78;229-235.

Khorsand A, Bayani M, Yaghobee S, Torabi S et al. Evaluation of salivary leptin levels in healthy subjects and patients with advanced periodontitis. J of Dent. 2016;13(1);1-9.

Umeki H, Tokuyama S, Ide S, Okubo M, Tadokoro S, Tezuka M, Tatehara S, Satomura K. Leptin promotes wound healing in the oral mucosa. PLoS ONE 2014;9(7);1-9.

Frank S, Stallmeyer B, Kampfer H, Kolb N, Pfeilscifter J. Leptin enhances wound re-epithelialization and constitutes a direct function of leptin in skin repair. J Clin Invest. 2000;106;501-509.

Murad A, Nath A, Cha S, Demir E, Flores-Riveros J, et al. Leptin is an autocrine/paracrine regulator fo wound healing. FASEB J. 2003;17;1895-1897.

Stallmeyer B, Kampfer H, Podda M, Kaufmann R, Pfeilschifter J, et al. A novel keratinocyte mitogen: regulation of leptin and its functional receptor in skin repair. J Invest Dermatol. 2001; 117;98-105.

Paul R, Hassan M, Nazar H, Gillani S, Afzal N, Qayyum I. Effect of body mass index on serum leptin levels. J Ayub Med. Coll Abbottabad. 2011;23(3);40-43.

Lago R, Gomez R, Lago F, Gomez-Reino J, Gualillo O. Leptin beyond body weight regulation: current concepts concerning its role in immune function and inflammation. Cell Immunol. 2008;252(1-2);139-145.

Fantuzzi G, Faggioni R. Leptin in the regulation of immunity, inflammation and hematopoiesis. J Leukoc Biol. 2000;68;437-446.

Agrawal S, Gollapudi S, Gupta S. Leptin activates human B cells to secrete TNFα, IL-6 and IL-10 via JAK2/STAT3 and p38MAPK/ERK1/2 signaling pathway. J Clin Immunol. 2011;31;472-478.

Meyers J, McTiernan A, Ulrich C. Leptin and immune function: integrating the evidence. Nutr Res. 2005;25;791-803.

Von Bremen J, Wagner J, Ruf S. Correlation between body mass index and orthodontic treatment outcome. Angle Orthod. 2013;83;371-375.

Dilisiz A, Kilic N, Aydin T, Ates N, Zihini M, Bulut C. Leptin levels in gingival cervicular fluid during orthodontic tooth movement. Angle Orthod. 2010;80(3);504-509.

Alhashimi N, Frithiof L, Brudvik P, Bakhiet M. Orthodontic tooth movement and de novo synthesis of proinflammatory cytokines. Am J Orthod Dentofacial Orthop. 2001;119;307-312.

Lee K, Park Y, Yu H, Choi S, Yoo Y. Effects of continous and interrupted orthodontic force on interleukin-1β and prostaglandin E2 production in gingival cervicular fluid. Am J Orthod Dentofacial Orthop. 2004;125;167-168.

Thilander B, Rygh P, Reitan K. Tissue reactions in orthodontics. In: Graber TM, ed. Orthodontics: Current Principles and Techniques. St Louis, MO: CV Mosby 2000;117–156.

Javed F, Yu W, Thornton J, Colt E. Effect of fat on measurement of bone mineral density. Int J Body Compos Res. 2009;7;37.

Myers M, Leibel R, Seeley R, Schwartz M. Obesity and leptin resistance:distinguishing cause from effect. Trends Endocrinol Metab. 2010;21;643-651.

Abdalla Ibrahim M, Choo S. Salivary leptin level in young adult males and its association with anthropometric measurements, fat distribution and muscle mass. Europ Endocrinol. 2018;14(2);94-98.

Groschl M, Topf H, Kratzsch J, Dotsch J, Rascher W, et al. Salivary leptin induces increased expression of growth factors in oral keratinocytes. J Mol Endocrinol. 2005;34;353-366.

Petersen P, Ogava H. Strengthening the prevention of periodontal disease: the WHO approach. J Periodontol 2005;76(12);187-193.

Bozkurt F, Yetkin Ay Z, Sutcu R, Delibas N, Demirel R. Gingival cervicular fluid leptin levels in periodontitis patients with long-term and heavy smoking. J Periodontol. 2006;77(4);634-640.

Karthikeyan B, Pradeep A. Leptin levels in gingival cervivular fluid in periodontal health and disease. J Periodontal Res 2007;42(4);300-304.

Purwar P, Khan M, Mahdi A, Pandey S, Singh B, Dixit J, et al. Salivary and serum leptin concentrations in patients with chronic periodontitis. J Periodontol. 2015;86(4);588-594.

Sattari M, Joze Khaje Nouri B, Mouzeh M, Mehr Mofakhkham S, Haji Molla Hosseini M, Yeganeh F. The association between leptin and chronić periodontitis. J Dent Sch Shahid Beheshti Univ Med Sci. 2011;29(4);285-288.

Salvarajan S, Perumalsamy R, Emmadi P, Thiagarajan R, Namasivayam A. Association between gingival cervicular fluid leptin levels and periodontal status-A biochemical study on Indian patients. J Clin Diagn Res. 2015;9(5);48-53.

Aydin S, Halifeoglu I, Ozercan I, Erman F, Kilic N, Aydin S, et al. A comparison of leptin and gherlin levels in plasma and saliva of young healthy subjects. Peptides 2005;26(4);647-652.

Gangadhar V, Ramesh A, Thomas B. Correlation between leptin and the health of the gingiva: A predictor of medical risk. Indian J Dent Res. 2011;22(4);537-541.



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