Modifying effect of diabetes mellitus on the dynamics of parameters of free radical process intensity and antioxidant protection activity in the liver of rats with incomplete global cerebral ischemia-reperfusion

S. S. Tkachuk, M. A. Povar, O. V. Tkachuk, V. F. Myslytskyi, V. P. Gavaleshko


According to the WHO expert assessment diabetes mellitus (DM) will take the seventh position among leading causes of death in 2030 [1]. The most dangerous complications of DM becoming a cause of severe disability or mortality are acute cerebral disorders of ischemic genesis [2, 3], which in case of this pathology occur several times more frequently than in case of its absence [4, 5]. The causes of complicated development of cerebral ischemia-reperfusion against the ground of DM are being investigated actively, and pathogenesis of brain damage in case of this comorbid pathology appears to be the most studied, although the mechanisms of morphofunctional disorders of the parenchymal organs remain without attention of researchers, since they are touched upon only in several works [6, 7]. Meanwhile, even short hypoxic conditions of the brain are known to result in considerable pathohistological changes in the internal organs becoming a cause of disorders in their functional state and require correction [7, 8]. The most permanent and long changes occur in the liver even to the destruction of separate cells or their groups [9-11]. These changes in case of cerebral ischemia are initiated due to redistribution of blood flow and oxygen supply in favour of the brain, and therefore partial oxygen pressure in the abdominal organs decreases, and in the liver in particular [10].
From the other hand, metabolic disorders associated with diabetes mellitus are generally known to cause liver damage [12-14], therefore diabetic complications caused by cerebral ischemia-reperfusion should be logically expected to intensify morphofuncitonal changes in the organ. Meanwhile, we have not found investigations of the kind described in the scientific literature yet.
Objective and tasks: to examine the signs of oxidative and nitrosative stress in the liver of rats with diabetes mellitus complicated by cerebral ischemia-reperfusion.
In the liver of rats without DM 20 minute carotid ischemia with 1 hour reperfusion by the changes of the content of lipid peroxidation products and activity of antioxidant enzymes results in depression of the lipid peroxidation-antioxidant protection system. On the 12th day of observation diene conjugated content increases in the organ against the ground of increased activity of superoxide dismutase and glutathione peroxidase, which in general is indicative of a compensatory reaction character of the system. The indices of protein oxidative modification and nitrogen oxide metabolism in the liver of animals without diabetes at the early post-ischemic period remain unchanged, and on the 12th day of the experiment they increase, which confirms availability of oxidative and nitrosative stress at this period. With DM available reaction of all the above indices in the liver is absent both at the early and late post-ischemic periods, which characterizes a-reactivity of these biochemical parameters concerning cerebral ischemia-reperfusion.


diabetes mellitus, antioxidant protection, global cerebral ischemia-reperfusion

Full Text:



IDF Diabetes Atlas. 7th ed. [Internet]. Brussels; 2015 [cited 2017 Jul 9]. 144 р. Available from:

Ji R., Schwamm L.H., Pervez M.A., Singhal A.B. Ischemic stroke and transient ischemic attack in young adults: risk factors, diagnostic yield, neuroimaging, and thrombolysis. JAMA Neurol. 2013;70(1):51-7. doi: 10.1001/jamaneurol.2013.575.

Lackland D.T., Roccella E.J., Deutsch A.F., Fornage M., George M.G., Howard G., et al. Factors influencing the decline in stroke mortality: a statement from the American Heart Association/American Stroke Association. Stroke. 2014;45(1):315-53. doi: 10.1161/

Tanaka R., Ueno Y., Miyamoto N., Yamashiro K., Tanaka Y., Shimura H., et al. Impact of diabetes and prediabetes on the short-term prognosis in patients with acute ischemic stroke. J Neurol Sci. 2013;332(1-2):45-50. doi: 10.1016/j.jns.2013.06.010.

Lindley R.I., Wardlaw J.M., Whiteley W.N., Cohen G., Blackwell L., Murray G.D., et al. Alteplase for acute ischemic stroke: outcomes by clinically important subgroups in the Third International Stroke Trial. Stroke. 2015;46(3):746-56. doi: 10.1161/STROKEAHA.114.006573.

Tkachuk О. V., Tkachuk S. S., Myslytskyi V. F., Gavaleshko V. P., Yasinska O. V., Povar M. A., Boshtan S. V., Savchuk T. P. Content of protein oxidative modification products and nitrogen monoxide metabolites in the kidneys and myocardium of rats with streptozotocin-induced diabetes in dynamics of cerebral ischemia-reperfusion. Journal of Education, Health and Sport. 2018;8(7):545-550. eISNN 2391-8306.

Havaleshko V.P. Histolohichni zminy v nyrkakh pry eksperymental'nomu tsukrovomu diabeti, uskladnenomu nepovnoyu hlobal'noyu ishemiyeyu-reperfuziyeyu holovnoho mozku. Klinichna anatomiya ta operatyvna khirurhiya. 2012;11(3):62-5.

Tkachuk O.V., Tkachuk S.S., Havaleshko V.P. Strukturna reaktsiya miokarda u shchuriv zi streptozototsyn-indukovanym diabetom, uskladnenym nepovnoyu hlobal'noyu ishemiyeyu-reperfuziyeyu holovnoho mozku. Klinichna anatomiya ta operatyvna khirurhiya. 2013;12(2):48-53.

Ruziev A.Sh.U., Murotov O.U., Ibragimov U.K. Antioksidantnaja sistema subkletochnyh frakcij pecheni pri jeksperimental'nom insul'te. Vestnik Novosibirskogo gosudarstvennogo pedagogicheskogo universiteta. 2013;5(15):122-26.

Muscari A., Collini A., Fabbri E., Giovagnoli M., Napoli C., Rossi V., et al. Changes of liver enzymes and bilirubin during ischemic stroke: mechanisms and possible significance. BMC Neurol. 2014;14:122. doi: 10.1186/1471-2377-14-122.

Sajfieva H.D., Ibragimov U.K. Morfologicheskie izmenenija v pecheni pri jeksperimental'nom insul'te. Vestnik RGMU. Zhurnal Vserossijskogo Gosudarstvennogo medicinskogo universiteta. 2009;3:65-6.

Sharma P., Bodhankar S.L., Thakurdesai P.A. Protective effect of aqueous extract of Feronia elephantum correa leaves on thioacetamide induced liver necrosis in diabetic rats. Asian Pac. J. Trop. Biomed. 2012 Sep;2(9):691-5. doi: 10.1016/S2221-1691(12)60211-1.

Kohl T., Gehrke N., Schad A., Nagel M., Wörns M.A., Sprinzl M.F., et al. Diabetic liver injury from streptozotocin is regulated through the caspase-8 homolog cFLIP involving activation of JNK2 and intrahepatic immunocompetent cells. Cell Death Dis. 2013;4(7): e712. doi: 10.1038/cddis.2013.228.

Simões C., Domingues P., Ferreira R., Amado F., Duarte J.A., Vitorino R., et al. Remodeling of liver phospholipidomic profile in streptozotocin-induced diabetic rats. Arch. Biochem. Biophys. 2013;538(2):95-102. doi: 10.1016/

Mahalyas V.M, Mikhyeyev A.O., Rohovyy Yu.Ye. Suchasni metody eksperymental'nykh ta klinichnykh doslidzhen' tsentral'noyi naukovo-doslidnoyi laboratoriyi Bukovyns'koyi derzhavnoyi medychnoyi akademiyi. Chernivtsi; 2001. 42 s.



  • There are currently no refbacks.

Copyright (c) 2018 © The Author (s) 2018

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

POL-index 2011 - 2014

BASE 2011 - 2014

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

Find a library that holds this journal:

Journal Language(s): English 

PBN Poland



Redaction, Publisher and Editorial Office

Instytut Kultury Fizycznej Uniwersytet Kazimierza Wielkiego w Bydgoszczy, Institute of Physical Education Kazimierz Wielki University in Bydgoszcz, Poland 85-091 Bydgoszcz ul. Sportowa 2 Copyright by Instytut Kultury Fizycznej UKW w Bydgoszczy  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at

SIC Science citation index (calculated on the basis of TCI and Page Rank) 0

Russian Impact factor 0.16

Indexed in Index Copernicus Journals Master List.,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48

The InfoBase Index IBI Factor for the year 2015 is 3.56 in InfoBase


Universal Impact Factor 1.78 for year 2012. (

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ)

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski