Are genes of interleukins' 10 (RS1800872) and heat shock protein 70-2 (RS1061581) predictive markers of cytokines production in patients with enterocolitis

B. V. Syrota, L. P. Sydorchuk


Objective: to investigate the effect of 1267 AG gene polymorphism of heat shock proteins (HSP) family 70-2 (HSP70-2) and C-592A gene polymorphism of interleukin 10 (IL-10) on the level IL-1β, TNF- and IL-10 in the blood of patients with colienteritis.
Materials and methods: 95 patients suffering from Colienteritis participated in the study including 34.74% (33) men, and 65.26% (62) women, their average age was 38.66±3.11 years. Concentration of IL-1β, TNF- та IL-10 in the blood plasma was examined by means of immune-enzyme method. Polymorphism of HSP70-2 (rs1061581) and IL-10 (rs1800872) genes was studied by means of polymerase chain reaction (PCR) method. The control group included 30 practically healthy individuals correlated by their age and sex who were not infected by diseases of any localization during the last 6 months.
Results. Changes of immune-inflammatory body response in patients with colienteritis are characterized by an increased synthesis of the first generation pro-inflammatory IL-1β and TNFα on 40.93% and 44.44% (р<0.001) respectively against the ground of low production of anti-inflammatory IL-10 – on 37.30% (р<0.001) and are indicative of certain mechanisms of specific immune response formation: imbalance between a high activity of the immune cellular link at the expense of Тh1 lymphocytes and macrophages/monocytes, and insufficient activity of the humoral link due toТh2 system and B-lymphocytes activity.
Single-factor dispersive analysis confirmed the association between the gene promoter of heat shock protein HSP70-2 (1267AG) and increased level of IL-1β in the blood, more considerable in the carriers of GG-genotype on 45,05% (F=11.66; p<0.001), and decreased concentration of IL-10 (F=4.56; p=0.035), more considerable in the carriers of GG-genotype on 23.38% (р=0.031). On the contrary, promoter of IL-10 (C-592A) gene is associated only with decreased content of IL-10 (F=3.14, p=0.048), the most substantial in the carriers of mutation A-allele on 26.95% and 20.64% (р<0.05) respectively.
Conclusions: Availability of GG-genotype of HSP70-2 gene in patients with Colienteritis is associated with a higher content of IL-1β increase in the blood plasma and a low concentration of IL-10. Occurrence of A-allele of IL-10 gene in the genotype of patients with Colienteritis is characterized by the lowest IL-10 content.


Colienteritis, Coliescherichiosis, Gastroenterocolitis, genes HSP70-2, IL-10, markers, cytokines IL-1β, TNFα, IL-10

Full Text:



Desilets M, Deng Xi, Rao C et al. Genome-based Definition of an Inflammatory Bowel Disease-associated Adherent-Invasive Escherichia coli Pathovar [Electronic resource]. Inflamm Bowel Dis 2016; 22(1):1-12 Access mode:

Futosi K., Fodor S and Mocsai A. Neutrophil cells surface receptors and their intracellular signal transduction pathway. Int Immunopharmacol 2013; 17(3):638-650.

Di Carlo E., Forni G., Lollini P.L., Colomobo M.P., Modesti A. and Musiani P. The intriguing role of polymorphonuclear neutrophil in antitumor reactions. Blood 2001; 97:339-345.

Sydorchuk LP, Serdulets YuI, Sydorchuk AR et al. The polymorphism of matrilin-3 (rs77245812) and interleukin-10 (rs1800872) genes in osteo-arthritis patients with arterial hypertension, obesity and type 2 diabetes mellitus. Archives of the Balkan Medical Union 2017; 52(4): 422-429

Sydorchuk L, Bochkariova O, Andriyets O, Sydorchuk A, Bodnariuk O. Heat-shock protein 70-2 gene (1267A→G) in association with vulvovaginitis and cytokines levels in juvenile girls of Northern Bukovina. The Pharma Innovation Journal 2016; 5(3): 19-23

Onderdijk AJ, Baerveldt EM, Kurek D, Kant M, Florencia EF, Debets R, Prens EP. IL-4 Downregulates IL-1β and IL-6 and Induces GATA3 in Psoriatic Epidermal Cells: Route of Action of a Th2 Cytokine. J Immunol 2015; 195(4):1744-52. doi: 10.4049/jimmunol.1401740.

Bester J, Pretorius E. Effects of IL-1β, IL-6 and IL-8 on erythrocytes, platelets and clot viscoelasticity. Sci Rep 2016; 6:32188. doi: 10.1038/srep32188.

Iftoda OM, Sydorchuk LP. Cytokine mechanisms of immunological disorders in children of Bukovina with deafness depending on polymorphisms of connexin-26 CJB2 (rs80338939) and interleukin-4 (rs2243250) genes. J. Clin. Exp. Med. Res. 2016; 4 (1):27-35.

Sydorchuk L, Iftoda O, Sydorchuk A et al. Cytokines' cascade changes in children with hearing loss depending on gap junction protein beta 2 (C.35delG) and interleukin 4 (C-590T) genes polymorphism. The Pharma Innovation Journal 2015; 5(2): 22-27

Carrera-Bastos P, Picazo Ó, Fontes-Villalba M, Pareja-Galeano H, Lindeberg S, Martínez-Selles M et al. Serum Zonulin and Endotoxin Levels in Exceptional Longevity versus Precocious Myocardial Infarction. Aging Dis. 2018; 9(2):317-321. doi: 10.14336/AD.2017.0630.

Panasiukova OR, Kadan LP. Cytokines and tuberculosis (literature review) [FTP archive]. 2008. URL



  • There are currently no refbacks.

Copyright (c) 2018 © The Author (s) 2018

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.

Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

POL-index 2011 - 2014

BASE 2011 - 2014

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

Find a library that holds this journal:

Journal Language(s): English 

PBN Poland



Redaction, Publisher and Editorial Office

Instytut Kultury Fizycznej Uniwersytet Kazimierza Wielkiego w Bydgoszczy, Institute of Physical Education Kazimierz Wielki University in Bydgoszcz, Poland 85-091 Bydgoszcz ul. Sportowa 2 Copyright by Instytut Kultury Fizycznej UKW w Bydgoszczy  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at

SIC Science citation index (calculated on the basis of TCI and Page Rank) 0

Russian Impact factor 0.16

Indexed in Index Copernicus Journals Master List.,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48

The InfoBase Index IBI Factor for the year 2015 is 3.56 in InfoBase


Universal Impact Factor 1.78 for year 2012. (

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ)

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski