Content of protein oxidative modification products and nitrogen monoxide metabolites in the kidneys and myocardium of rats with streptozotocin-induced diabetes in dynamics of cerebral ischemia-reperfusion

O. V. Tkachuk, S. S. Tkachuk, V. F. Myslytskyi, V. P. Gavaleshko, O. V. Yasinska, M. A. Povar, S. V. Boshtan, T. P. Savchuk


Introduction. Acute disorders of cerebral circulation similar to diabetes mellitus (DM) cause long-term multiple organ effects, although pathogenesis of internal organs injury in association with DM and cerebral ischemia-reperfusion has not been investigated completely.
The aim of the study. To study the dynamics of the content of protein oxidative modification and metabolites in the myocardium and kidneys of rats with DM complicated by cerebral ischemia-reperfusion.
Results. The content of protein oxidative modification products in the tissues of the kidneys and myocardium and the content of nitrogen monoxide metabolites in the cortical and medullary substance of the kidneys were found to decrease in the early ischemic-reperfusion period in rats without DM. In rats with DM early post-ischemic changes are restricted by the increase of nitrogen oxide metabolites content in the renal medullary substance. Total remote changes of the examined parameters are found in the myocardium of rats without DM and the medullary substance of the kidneys in rats with DM.
Conclusions. Diabetes mellitus eliminates or restricts the reaction of the examined indices peculiar for control animals in both periods of observation in all the examined tissues except the medullary substance of the kidneys on the 12th day of the post-ischemic period.


diabetes mellitus, nephropathy, cardiomyopathy, cerebral ischemia-reperfusion.

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Aneja A., Tang W.H., Bansilal S., Garcia M.J., Farkouh M.E. Diabetic cardiomyopathy: insights into pathogenesis, diagnostic challenges, and therapeutic options. Am. J. Med. .2008; 121(9):748-57.

Ernande L., Derumeaux G. Diabetic cardiomyopathy: myth or reality? Arch. Cardiovasc. Dis. 2012; 105(4): 218-25.

Stevens L.A., Li S., Kurella Tamura M., Chen S.C., Vassalotti J.A., Norris K.C., Whaley-Connell A.T., Bakris G.L., McCullough P.A.Sowers Сomparison of the CKD epidemiology collaboration (C KD-EPI) and modification of diet in renal disease (MDRD) study equations: prevalence of and risk Factors for Diabetes Mellitu s in CKD in the Kidney Early Evaluation Program (KEEP). Am. J. Kidney Dis. 2011; 57(Is.3, Suppl. 2): 24-31.

Shao D., Liu J., Ni J., Wang Z., Shen Y., Zhou L., et al. Suppression of XBP1S Mediates High Glucose-Induced Oxidative Stress and Extracellular Matrix Synthesis in Renal Mesangial Cell and Kidney of Diabetic Rats. PLoS One. 2013; 8(2): e56124.

Boghdady N.A., Bard G.A. Evaluation of oxidative stress markers and vascular risk factors in patients with diabetic peripheral neuropathy. Cell. Biochem. Funct. 2012; 30(4): 328–34.

Mild oxidative damage in the diabetic rat heart is attenuated by glyoxalase-1 overexpression / O. Brouwers, J.M. de Vos-Houben, P.M. Niessen [et al.] // Int. J. Mol. Sci. – 2013. – Vol.14, №8. – Р. 15724-15739.

Falcão-Pires I., A.F. Leite-Moreira. Diabetic cardiomyopathy: understanding the molecular and cellular basis to progress in diagnosis and treatment. Heart Fail. Rev. 2012; 17(3): 325-44.

Sedeek M., Callera G., Montezano A. Critical role of Nox4-based NADPH oxidase in glucose-induced oxidative stress in the kidney: implications in type 2 diabetic nephropathy. Am. J. Physiol. Renal Physiol. 2010; 299(№6): 1348-58.

Muscari A., Collini A., Fabbri E., Giovagnoli M., Napoli C., Rossi V., et al. Changes of liver enzymes and bilirubin during ischemic stroke: mechanisms and possible significance. BMC Neurol. 2014;14(122). doi: 10.1186/1471-2377-14-122.

Chiu N.L., Kaiser B., Nguyen Y.V., Welbourne S., Lall C., Cramer S.C. The Volume of the Spleen and its Correlates after Acute Stroke. J. Stroke Cerebrovasc. Dis. 2016; 25(12): 2958–61.

Hassan Y., Aziz N.A., Al-Jabi S.W., Looi I. Evaluation of antihypertensive therapy among ischemic stroke survivors: impact of ischemic heart disease. J. Cardiovasc. Pharmacol. Ther. 20104; 15(3): 282-88.

Banerjee A., Lim C.C., Silver L.E., Welch S.J. Familial history of stroke is associated with acute coronary syndromes in women. Circ. Cardiovasc. Genet. 2011; 4(1). Р.9-15.

Rashidi A., Sehgal A.R., Rahman M., O'Connor A.S. The case for chronic kidney disease, diabetes mellitus, and myocardial infarction being equivalent risk factors for cardiovascular mortality in patients older than 65 years. Am. J. Cardiol. 2008; 102(12): 1668-73.

Cianciolo G., De Pascalis A., Di Lullo L., Ronco C., Zannini C., La Manna G. Folic Acid and Homocysteine in Chronic Kidney Disease and Cardiovascular Disease Progression: Which Comes First? Cardiorenal. Med. 2017; 7(4): 255–66.

Tkachuk, S.S., Lenkov, A.M. Ekspresiia bilkiv Hif-1α, р53 ta Bcl-2 v golovnomu mozku za umov dvobichnoi karotidnoi ishemii-reperfuzii na tli tsukrovogo diabetu v samtsiv-shchuriv. Klinichna ta eksperimentalna patologiia. 2010; 2(32): 111–113.

Skibo G.G. Ispolzovanie razlichnikh eksperimentalnikh modelei dlia izucheniia kletochnikh mechanizmov іshemicheskogo porazheniia mozga. Patologija. 2004; 1(1): 22-30.

Mahalyas VM, Mikhyeyev AO, Rohovyy YuYe. Suchasni metody eksperymental'nykh ta klinichnykh doslidzhen' tsentral'noyi naukovo-doslidnoyi laboratoriyi Bukovyns'koyi derzhavnoyi medychnoyi akademiyi. Chernivtsi; 2001. 42 s.



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