Background. Previously, we have shown that the weekly load of rats with water-salt solutions of different chemical compositions causes both general and specific reactions of the parameters of metabolism. The purpose of this study is to identify under these conditions specific neuroendocrine and immune responses. Materials and methods. Experiment was performed on 58 healthy female Wistar rats 240-290 g divided into 6 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Instead, the other rats received the same tap water as well as waters Sophiya, Naftussya, Gertsa and its artificial salt analogue through the probe at a dose of 1,5 mL/100 g of body mass for 6 days. The day after the completion of the drinking course in all rats some neuroendocrine and immune parameters were registered. Results. The method of discriminant analysis revealed 29 parameters of the neuroendocrine-immune complex (10 of them reflect the neuroendocrine regulation, 4 thymus mass and thymocytogram elements, 5 elements of splenocytogram, 10 elements of immunocytogram and leukocytogram of blood and parameters of phagocytosis), according to which the reaction on various water-salt loads are identified with an accuracy of 98.3%. Conclusion. The peculiarities of the reactions of the parameters of the neuroendocrine-immune complex are due to the content of water in sulfate, bicarbonate and magnesium, as well as organic carbon and nitrogen.

Water-salt loads; neuroendocrine and immune parameters; female rats

Baevskiy RM, Kirillov OI, Kletskin SZ. Mathematical Analysis of Changes in Heart Rate by Stress [in Russian]. Moskva: Nauka; 1984: 221 p.

Bianco C. Population of lymphocytes bearing a membrane receptor for antigen-antibody complex. J Exp Med. 1970; 134(4): 702-720.

Bilas VR, Popovych IL. Role of microflora and organic substances of water Naftussya in its modulating influence on neuroendocrine-immune complex and metabolism [in Ukrainian]. Medical Hydrology and Rehabilitation. 2009; 7(1): 68-102.

Douglas SD, Quie PG. Investigation of Phagocytes in Disease. Churchil; 1981: 110 p.

Goryachkovskiy АМ. Clinical Biochemistry [in Russian]. Odesa: Astroprint; 1998: 608 p.

Gozhenko AI, Zavidnyuk YV, Sydliaruk NI, Mysula IR, Klishch IM, Zukow W, Popovych IL, Korda MM. Features of metabolic reactions to various water-salt loads in female rats. Journal of Education, Health and Sport. 2018; 8(4): 496-518.

Jondal M, Holm G, Wigzell H. Surface markers on human T and B lymphocytes. I. A large population of lymphocytes forming nonimmune rosettes with sheep red blood cells. J Exp Med. 1972; 136(2): 207-215.

Klecka WR. Discriminant Analysis [trans. from English in Russian] (Seventh Printing, 1986). In: Factor, Discriminant and Cluster Analysis. Moskwa: Finansy i Statistika; 1989: 78-138.

Kozyavkina OV, Kozyavkina NV, Gozhenko OA, Gozhenko AI, Barylyak LG., Popovych IL. Bioactive Water Naftussya and Neuro-Endocrine-Immune Complex [in Ukrainian]. Kyiv: UNESCO-SOCIO; 2015: 349 p.

Lapovets’ LYe, Lutsyk BD. Handbook of Laboratory Immunology [in Ukrainian]. Lviv; 2002: 173 p.

Limatibul S, Shore A, Dosch HM, Gelfand EW. Theophylline modulation of E-rosette formation: an indicator of T-cell maturation. Clin Exp Immunol. 1978; 33(3): 503-513.

Polovynko IS, Zajats LM, Zukow W, Yanchij RI, Popovych IL. Quantitative evaluation of integrated neuroendocrine and immune responses to chronic stress in rats male. Journal of Education, Health and Sport. 2016;-6(8): 154-166.

Popovych IL. Functional interactions between neuroendocrine-immune complex in males rats [in Ukrainian]. Achievements of Clinical and Experimental Medicine. 2008; 2(9): 80-87.

Popovych IL. The concept of neuroendocrine-immune complex (Review) [in Russian]. Medical Hydrology and Rehabilitation. 2009; 7(3): 9-18.

Popovych IL, Ivassivka SV. Role of organic substances of water Naftussya in its physiological activity [in Ukrainian]. Medical Hydrology and Rehabilitation. 2009; 7(2): 6-26.

Popovych IL, Vis’tak HI, Gumega MD, Ruzhylo SV. Vegetotropic Effects of Bioactive Water Naftussya and their Endocrine-Immune, Metabolic and Hemodynamic Accompaniments [in Ukrainian]. Kyiv: UNESCO-SOCIO; 2014: 163 p.

Uchakin PN, Uchakina ON, Tobin BV, Ershov FI. Neuroendocrine immunomodulation [in Russian]. Vestnik Ross AMN. 2007; 9: 26-32.

Zajats LM, Polovynko IS, Zukow W. Features neuro-endocrine support diversity of immune responses to chronic stress in male rats. Journal of Education, Health and Sport. 2017; 7(3): 97-105.

Zavidnyuk YV, Mysula IR, Klishch IM, Zukow W, Popovych IL, Korda MM. Non-specific metabolic, neuroendocrine and immune reactions to various water-salt loads in female rats. In: XVII reading the name of VV Podvysotsky. Bulletin of Sci. Conf. (May 24-25, 2018). Odesa: UkrSRI of Transport Medicine, 2018: 34-36.

Zavidnyuk YV, Mysula IR, Klishch IM, Zukow W, Popovych IL, Korda MM. General non-specific metabolic, neuroendocrine and immune reactions to various water-salt loads in female rats. Journal of Education, Health and Sport. 2018; 8(3): 513-524.