Features of metabolic reactions to various water-salt loads in female rats

Anatoliy I Gozhenko, Yuriy V Zavidnyuk, Nataliya I Sydliaruk, Igor R Mysula, Ivan M Klishch, Walery Zukow, Igor L Popovych, Mykhaylo M Korda


Background. In the previous article we reported that screening registered parameters of water-salt, nitrous and lipid metabolism as well as the neuroendocrine-immune complex found 42 among them who in rats subjected to various water-salt loads, significantly different from that of intact rats, but on average the same group of animals that received liquids with different mineralization and chemical composition. The purpose of this article is to find out the features of the reactions of the parameters of metabolism. Materials and methods. Experiment was performed on 58 healthy female Wistar rats 240-290 g divided into 6 groups. Animals of the first group remained intact, using tap water from drinking ad libitum. Instead, the other rats received the same tap water as well as waters Sophiya, Naftussya, Gertsa and its artificial salt analogue through the probe at a dose of 1,5 mL/100 g of body mass for 6 days. The day after the completion of the drinking course in all rats the parameters of water-salt, nitrous and lipid metabolism were registered. Results. Found that 16 metabolic parameters the maximum deviates from the level of intact rats under the influence of the salt analogue of Gertsa water, a smaller, but tangible effect is made by the Gertsa native water, even less effective waters Sofiya and Naftussya, instead of ordinary water is almost ineffective in relation to these metabolic parameters. The other 19 parameters deviates to a maximum extent from the reference level after the use of water Naftussya, fresh water is less effective, whereas quasi-isotonic liquids are practically inactive for these parameters. The remaining 13 parameters in animals that use normal water, deviates from intact control to the same extent as in the previous pattern, which, apparently, is also due to the stressful effects of the load course. Both Naftussya and Gertsa water and its salt analogue prevent the stress deviations of these parameters. Instead, by consumption of water Sofiya stresses deviations of these parameters is reversed. The method of discriminant analysis revealed 33 variables (among them 8 refer to plasma/erythrocytes electrolytes, 7 to electrolytes of urine, to other metabolic parameters of plasma 5 and urine 9, as well as glomerular filtration, canalicular reabsorption, diurese and urine osmolarity), the totality of which the metabolic reactions to various water-salt loads are identified (recognized) with an accuracy of 98,3%. Conclusion. The features of the reactions of the parameters of metabolism are due to the content in waters NaCl, SO42- as well as organic carbon and nitrogen.


Water-salt loads; water-salt; nitrous and lipid metabolism; female rats.

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Bilas VR, Popovych IL. Role of microflora and organic substances of water Naftussya in its modulating influence on neuroendocrine-immune complex and metabolism [in Ukrainian]. Medical Hydrology and Rehabilitation. 2009; 7(1): 68-102.

Chebanenko OI, Chebanenko LO, Popovych IL. Variety Balneoeffects of Factors Spa Truskavets’ and their Forecast [in Ukrainian]. Kyiv: UNESCO-SOCIO; 2012: 496 p.

Chebanenko OI, Flyunt IS, Popovych IL, Balanovs’kyi VP, Lakhin PV. Water Naftussya and hydro-mineral exchange [in Ukrainian]. Kyiv: Naukova dumka; 1997: 141 p.

Goryachkovskiy АМ. Clinical Biochemistry [in Russian]. Odesa: Astroprint; 1998: 608 p.

Gozhenko AI, Dolomatov SI, Shumiliva PA, Topor EA, Pyatenko VA, Badiin IYu. The effect of osmotic stresses on the functional state of healthy kidneys [in Russian]. Nefrologiya. 2004; 8(2): 44-48.

Ivassivka SV. Biological Active Substances of Water Naftussya, their Genesis and Mechanisms of Physiological Effects [in Ukrainian]. Kyiv: Naukova dumka; 1997: 110 p.

Ivassivka SV, Bubnyak AB, Kovbasnyuk ММ, Popovych IL. Genesis and role of phenols in waters from Naftussya layer [in Ukrainian]. In: Problems of pathology in experiment and clinic. Scientific works of Drohobych Medical Institute. Vol. XV. Drohobych: 1994: 6-11.

Іvassivka SV, Popovych IL, Aksentijchuk BI, Bilas VR. Nature of Balneofactors of Water Naftussya and Essence its Curative and Prophilactive Effects [in Ukrainian]. Truskavets’: Truskavets’kurort; 1999: 125 p.

Іvassivka SV, Popovych IL, Aksentijchuk BI, Flyunt IS. Physiological Activity of Uric Acid and its Role in the Mechanism of Action of Water Naftussya [in Ukrainian]. Kyiv: Computerpress; 2004: 163 p.

Klecka WR. Discriminant Analysis [trans. from English in Russian] (Seventh Printing, 1986). In: Factor, Discriminant and Cluster Analysis. Moskwa: Finansy i Statistika; 1989: 78-138.

Kozyavkina OV, Kozyavkina NV, Gozhenko OA, Gozhenko AI, Barylyak LG., Popovych IL. Bioactive Water Naftussya and Neuro-Endocrine-Immune Complex [in Ukrainian]. Kyiv: UNESCO-SOCIO; 2015: 349 p.

Popovych IL. Factor and canonical analysis parameters of neuro-endocrine-immune complex, metabolism and erosive and ulcerative injuries of stomach mucosa in rats under acute water-immersion stress [in Ukrainian]. Medical Hydrology and Rehabilitation. 2007; 5(2): 68-80.

Popovych IL, Ivassivka SV. Role of organic substances of water Naftussya in its physiological activity [in Ukrainian]. Medical Hydrology and Rehabilitation. 2009; 7(2): 6-26.

Popovych IL, Vis’tak HI, Gumega MD, Ruzhylo SV. Vegetotropic Effects of Bioactive Water Naftussya and their Endocrine-Immune, Metabolic and Hemodynamic Accompaniments [in Ukrainian]. Kyiv: UNESCO-SOCIO; 2014: 163 p.

Yaremenko МS, Ivassivka SV, Popovych AI, Bilas VR et al. Physiological Bases of Curative Effect of Water Naftussya [in Russian]. Kyiv: Naukova dumka; 1989: 144 p.

Zavidnyuk YV, Mysula IR, Klishch IM, Zukow W, Popovych IL, Korda MM. General non-specific metabolic, neuroendocrine and immune reactions to various water-salt loads in female rats. Journal of Education, Health and Sport. 2018; 8(3): 513-524.

Dolomatov, S. I., Gozhenko, A. I., Moskalenko, T. Ja., Reutov, V. P., & Dolomatova, E. A. Vlijanie askorbinovoj kisloty na pochechnyj transport jendogennyh nitratov i nitritov u cheloveka. [Effect of ascorbic acid on renal transport of endogenous nitrates and nitrites in humans]. Jeksperimental'naja i klinicheskaja farmakologija. 2005;68(1): 50-52. DOI https://doi.org/10.30906/0869-2092-2005-68-1-50-52

Gozhenko, AI. Rol" oksydu azotu v molekulyarno klitynnyx mexanizmax funkciyi nyrok. [Role of nitric oxide in molecular cellular mechanisms of renal function]. Ukrayins"kyj bioximichnyj zhurnal. 2002;74(4a): 96.

Gozhenko, A. I. Ocherki teorii bolezni. [Essays on the theory of the disease]. 2010. Odessa.

Reutov V.P., Sorokina E.G., Gozhenko A.I., Kosicyn N.S., Gurin V.N. Cikl oksida azota kak mehanizm stabilizacii soderzhanija no i produktov ego prevrashhenija v organizme mlekopitajushhih. [Nitric oxide cycle as a mechanism for stabilizing NO content and products of its transformation into mammalian organisms]. Aktual'nі problemi transportnoї medicini. 2008;1(11): 22-28.

Bileckij, S. V., & Gozhenko, A. I. (2007). Gipoksicheski-giperkapnicheskie trenirovki v kardiologii. [Hypoxic-hypercapnic exercises in cardiology]. 2007. Chernovcy.

Gozhenko A.I., Gozhenko E.A. Sanogenez - teoreticheskaja osnova medicinskoj reabilitacii. [Sanogenesis is the theoretical basis of medical rehabilitation]. Medichna gіdrologіja ta reabіlіtacіja. 2007;5(2): 4-7.

Gozhenko A.I., Lisobej V.A., Psjadlo Je.M., Badjuk N.S. Professional'naja obuslovlennost' formirovanija psihofiziologicheskih osobennostej u rabotnikov transporta. [The professional conditioning of the formation of psychophysiological features of transport workers]. Aktual'nі problemi transportnoї medicini. 2008;3(13): 8-14.

DOI: http://dx.doi.org/10.5281/zenodo.1258073


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