The processes of apoptosis and telomerase activity in endometrial cells under different morphological conditions

Z. V. Chumak, N. V. Shapoval

Abstract


Aging and apoptosis are two mechanisms of a powerful anti - cancer defense. The apoptosis inhibitor is Bcl-2 gene. It expresses in transformed cells, blocks programmed death, promotes the survival of malignant ones. Under experimental conditions, when the Bcl-2 gene was transfected into the endothelial aging cells of mice, there was a decrease in mitochondrial oxidative stress, renewal of the potential of mitochondrial membranes, and improvement of angiogenesis. The objective: to study the expression of the Bcl-2 marker and telomerase activity in endometrial tissue with hyperplasia. Materials and methods. The endometrial tissue samples: 54 with hyperplastic process and 14 patients with a biphasic menstrual ovulatory cycle. Immunohistochemical reactions, real-time PCR with SYBR Green (RQ-TRAP) dye by Wege et al. In endometrial cells with complex non-atypical hyperplasia take place initial disturbances in the processes of programmed cellular death. In complex hyperplasia with atypia in endometrial cells there is inhibition of apoptosis, which can be regarded as a trigger mechanism in the development of cellular atypia. The statistically significant parallelism between the processes of telomerase reactivation in endometrial cells at complex atypical hyperplasia and the existence of telomerase activity in 85% of tumors allows to appraise it as a predictor of oncological process.

Keywords


apoptosis, p53, Bcl-2, telomeres, telomerase, malignancy, senescence

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References


Berestyanaya AN, Grodzinsky DM The role of telomeres in the process of cell aging // Naukovy Vіsnik Uzhgorodskogo yniversitetu. Ser. Biology. - 2012. - V. 33. - P. 5-16

Egorov EE Telomeres, telomerase, carcinogenesis and health measure // Clinical oncohematology. - 2010. - V 3. - No. 2. - P. 184 - 197

Expression of genes that regulate apoptosis in different types of endometrial hyperplasia and endometrial adenocarcinoma / Chernukha GE, Dumanovskaya MR, Burmenskaya OV, et al. // Obstetrics and gynecology .- 2013.- № 1. - P. 11-14.

Telomerase: methods for determining activity / D.A. Skvortsov, M.E. Zvereva, O. V. Shpanchenko, et al.// Acta Nature.- 2011. - V.1.- №1 (8) .- P. 51 - 72.

Frolova I.I. Risk factors for endometrial cancer // Questions of gynecology, obstetrics and perinatology .- 2010.- N.1. - P. 57 - 66.

Telomerase functions: telomere extension and not only / M.P. Rubtsova, D. P. Vasilkova, A.N. Malyavko, et al. // Acta Nature.- 2012.- V.4.- №2 (13) .- P.44-61.

Chumakov P.M. Protein p53 and its universal functions in a multicellular organism. // Uspekhi biologicheskoi khimii. - 2007.- V. 47.- P. 3 - 52.

Aberrant Telomerase Expression in the Endometrium of Infertile Women with Deep Endometriosis/ Fernanda A. Mafra, Denise M. Christofolini, Viviane Cavalcanti et al. // Archives of Medical Research.-2014.- 45.- 31-35.

Immunohistochemical analysis of steroid receptors, proliferation markers, apoptosid related molecules, and gelatinases in non-neoplastic and neoplastic endometrium./ Amalinei C., Cianga C., Balan R. et al.// Ann. Anat.-2011; 193(1): 43-45.

Pat, Monaghan. Organismal stress, telomeres and life histories// The Journal of Experimental Biology.- 2014.- 217.- Р. 57-66.

Preoperative evaluation of P53 and Bcl-2 over expression in clinical stage 1 endometrial carcinoma and their correlation with surgical-pathological data and prognosis of patients/ Emad A. Fyallah, Reda A. Hemida, Kamal I. Anwar et al.// Open Journal of Obstetrics and Gynecology.-2011.-№1.-P.55-63.

The use of an immunocytochemical double-labeling staining can display the distribution of Bcl-2/Ki67 cells in endometrial adenocarcinomas as well as in normal endometrium/ Mourtzikou A., Kosmas K., Marouga A. et al.// Clin Lab.- 2012.- №58(1-2).- P.133-144.




DOI: http://dx.doi.org/10.5281/zenodo.1256846

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