A study on the lipid peroxidation status and the antioxidant system in rats at experimental diabetic retinopathy

Volodymyr Volodymyrovych Semenko, Valeriy Mykolayovych Serdyuk, Ivan Volodymyrovych Savytskyi

Abstract


Activation of free radical oxidation is one of the key elements of the diabetes mellitus pathogenesis that leads to structural and functional disruption of membranes. Oxidative stress and imbalance between its intensity and the antioxidant system condition, comply with the data obtained from the literature, play an important role in the development of this disease complications. The defect of β-cells of Langerhans islets caused by hyperglycemia enhanced oxidative stress, is also substantially affect the progression of diabetes and its complications.

The aim of this work is to study the status of lipid peroxidation and the antioxidant system at experimental diabetes, as well as to study the effectiveness of arginine corrective action.

Wistar outbreeding white rats were used in the study, with the weight of 180-200 g. Comply with objectives of the work, animals were ranked into 3 groups: group 1– 20 animals that were not subjected to any influence; they served as control; group 2 – 30 animals in which diabetes mellitus was simulated; group 3 – 30 animals were aimed to receive a 7 % arginine solution, against the backdrop of diabetes.

Diabetes was modeled with an intraperitoneal three times delivery of alloxan at a dose of 7.5 ml, with 5 days interval. Delivery of alloxan have been carried out with the animals free drinking of 5% fructose solution. The experiment lasted 30 days. Scientists witnessed 100% survival of experimental rats while applying this model.

Activation of lipid peroxidation was detected with the alloxan diabetes model, as evidenced by an increase in the MDA and diene conjugates content in blood serum. There is a decrease in the catalase and superoxide dismutase activity at the experimental diabetes. Corrective action of arginine has led to a decrease in lipid peroxidation (LPO) processes and to the antioxidant system activation.


Keywords


experimental diabetes mellitus, alloxanic model, oxidization of peroxide of lipids, antioxidant system, dien conjugates, malonic dialdehyde, superoxideddismutasa, katalasa

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References


Zenkov N.K. Oksidativnyy stress. Biokhimicheskiy i patofiziologicheskiy aspekty / N.K. Zenkov – M.: MAIK "Nauka / Interperiodika", 2001. – 343 s

Mozheyko L.A. Eksperimental'nyye modeli dlya izucheniya sakharnogo diabeta / L.A. Mozheyko // Zhurnal Grodnenskogo gosudarstvennogo meditsinskogo universiteta. – 2013. – №3. – S.26 – 29.

Kubatiyev A.A Perekisi lipidov i tromboz / A.A. Kubatiyev, S.V. Andreyev // Byulleten' eksperimental'noy biologii i meditsiny. – 1979. – № 5 – S. 414 – 17.

Kamyshnikov V.S. Spravochnik po kliniko-biologicheskoy laboratornoy

diagnostike / V.S. Kamyshnikov — Minsk, 2000. — 219 s.

Balabolkin M.I. Rol' glikirovaniya belkov, okislitel'nogo stressa v patogeneze sosudistykh oslozhneniy pri sakharnom diabete / M.I. Balabolkin // Sakharnyy diabet. — 2002. — № 4. — S. 8 – 16.

Malyshev A.V. Biokhimicheskiye izmeneniya steklovidnogo tela pri razlichnykh vidakh vitreoretinal'noy patologi / A.V. Malyshev // Fundamental'nyye issledovaniya. – 2013. –№ 9. – S. 195 – 201.

Nikolayeva G.V. Formirovaniye autoregulyatsii krovotoka setchatki u nedonoshennykh novorozhdennykh / G.V. Nikolayeva // Rossiyskaya detskaya oftal'mologiya. – 2013. – № 1. –S.13 – 16.

Skripets P.P. Prognozirovaniye i profilaktika tyazhelykh iskhodov retinopatii nedonoshennykh: diss. … kand. med.nauk: 14.00.08 Skripets Petr Petrovich. – M., 2003. – 114 s.

Lee J.W.Future applications of antioxidants in premature infants / J.W. Lee, J.M. Davis // Curr. Opin. Pediatr. – 2011. – № 23 (2). – Р. 161 – 166.

Mataftsi A. Mediator sinvolved in retinopathy of prematurity and emerging therapeutic targets / A. Mataftsi, S.A. Dimitrakos, G.G. Adams // Early Hum. Dev. – 2011. – № 87(10). – Р.683 – 690.

Fayzullina A.S. Znacheniye sostoyaniya antioksidantnoy zashchity u detey s retinopatiyey nedonoshennykh / A.S. Fayzullina, G.KH. Zaynutdinova // Tochka zreniya. Vostok - Zapad. – 2016. – № 4. – S.59 – 62.

Li S.Y. Hypoxia-induced oxidative stress in ischemic retinopathy. Oxidative Medicine and Cellular Longevity / S.Y. Li, Z.J. Fu., A.C. Lo [Электронный ресурс]. – 2012. Режим доступа: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3483772/.

Srubilin D.V. Rol' nitroksidergicheskoy sistemy v regulyatsii okislitel'nogo stressa v pecheni u krys s eksperimental'nym peritonitom / D.V. Srubilin, D.A. Yenikeyev, V.A. Myshkin // Fundamental'nyye issledovaniya. – 2014. – № 10-4. – S. 724 – 731.

McCord J.M. Superoxide Dismutase an Enzymic Function for Erythrocuprein (Hemocuprein) / J. M. McCord, I. Fridovich // The Journal of Biolooical Ciiemistry. – 1969. – Vol.244. – №22. – P. 6049 – 6055.

Reutov V.P. NO sintaznaya i nitritreduktaznaya komponenty tsikla oksida azota / V.P. Reutov, Ye.G. Sorokina // Biokhimiya. – 1998. – T. 63. – № 7. – S. 1029–1040

Beckman J.S. Oxidative chemistry of peroxynitrite / J.S. Beckman, J. Chen, H. Ischiropoulos [et al.] // Methods Enzymol. – 1994. – № 233. – P. 229 – 240.

Bondarenko O.N. Biologicheskaya rol' oksida azota pri sakharnom diabete / O.N. Bondarenko, G.R. Galstyan, M.B. Antsiferov [i dr.] // Sakharnyy diabet. – 2002. – №2. – S. 56 – 63.

Tovmasyan M.S. Antistressorn'y effekt vnutrivenno vvedennogo L-Arginina pri povrezhdenii pecheni krys, vyzvannnom ishemiyey-reperfuziyey [Elektronnyy resurs] / M.S. Tovmasyan // Voprosy teoreticheskoy i klinicheskoy meditsiny. – 2008. – №2. Rezhim dostupa: www.med-practic.com

Rhee J.E. The effects of antioxidants and nitric oxide modulators on hepatic ischemic-reperfusion injury in rats / J.E. Rhee, S .E. Lung, S.D. Shin [et al.] // J.Korean Med.Sci. – 2002. –№ 17(4) . – С. 502 – 506.

Dorokhina L.V. Prooksidantno-antioksidantnoye ravnovesiye u krys pri gipotermii v usloviyakh korrektsii L-arginin-NO sistemy / L.V. Dorokhina, V.V. Zinchuk // Vestsí AN RB. Ser. Bíyal.nav. – 2000. – №4. – S.87 – 90.

Bliznetsova G.N. Sostoyaniye antioksidantnoy sistemy v usloviyakh stimulyatsii L-argininom produktsii oksida azota pri toksicheskom povrezhdenii pecheni // Materialy mezhdunarodnoy nauchno-prakticheskoy konferentsii «Svobodnyye radikaly, antioksidanty i zdorov'ye zhivotnykh». Voronezh: VGU, 2004. – S. 17-21.

Gabrilyan N.I. Opredeleniye srednikh molekul skrining-metodom. Spektrofotometricheskoye opredeleniye soderzhaniya GPL v plazme krovi / N.I. Gabrilyan, B.V. Gavrilov, M.N. Mishkorudnaya // Lab. delo. – 1983. – № 3. – S. 33 – 36.

Chevari S. Opredeleniye antioksidantnykh parametrov krovi i ikh diagnosticheskoye znacheniye v pozhilom vozraste / S. Chevari, T. Andel, YA. Shtrener // Laboratornoye delo. – 1991. – №10. – S. 9 – 13.

Kostyuk V.A. Prostoy i chuvstvitel'nyy metod opredeleniya aktivnosti SOD, osnovannyy na reaktsii okisleniya kvertsetina / V.A. Kostyuk, A.N. Potapova, ZH.V. Kovaleva // Voprosy meditsinskoy khimii. – 1990. – № 2. – S. 88 – 91.

Shaffer J.P. Multiple Hypothesis Testing / J.P. Shaffer //Annual Review of Psychology. – 1995. – № 46. – Р. 561 – 584.

Semenko V.V. Rozrobka eksperymentalʹnoyi aloksanovoyi modeli tsukrovoho diabetu/ V.V. Semenko, V.M. Serdyuk, Y.V. Savytskyy // Mezhdunarodnyy éndokrynolohycheskyy zhurnal. – 2017. – T.13. – №4. – S.276 – 280.

S. Glants. Mediko-biologicheskaya statistika: per. s angl. / Glants S. – M.: Praktika, 1998. - 459 s.

Matveyeva I.I. Oksid azota i endogennaya intoksikatsiya u onkologicheskikh bol'nykh / I.I. Matveyeva, G.N. Zubrikhina, E.G. Gorozhanskaya [i dr.] // Vesnik RONTS im. N.N.Blokhina RAMN. – 2008. – T.19. – № 14. – S.55 – 61.

Méndez J.D. L-arginine and polyamine administration protect cells against alloxan diabetogenic effect in Sprague-Dawley rats / J.D. Méndez, R. De Haro-Hernández // Biomed Pharmacother. – 2005. –№ 59. – Р. 283 – 289

Vasilijevic A. Beneficial effects of L-arginine–nitric oxide-producing pathway in rats treated with alloxan / A. Vasilijevic, B. Buzadzic, A. Korac [et al] // J. Physiol. – 2007. – Vol. 5849 (3). – P. 921 – 933.

Meretsʹkyy V. M. Porushennya lipidnoho ta vuhlevodnoho obminu i metody yikh korektsiyi pry eksperymentalʹnomu tsukrovomu diabeti / V. M. Meretsʹkyy // Medychna khimiya. – 2007. –T9. – №3. – S.83 – 86.

Palʹchykova N. O. Hormonalʹno-biokhimichni osoblyvosti alloksanovoyi ta streptozototsynovoyi modeley eksperymentalʹno diabetu / N. O. Palʹchykova, N. V. Kuznetsova, O. I. Kuzʹmynova [ta in] // Byuletenʹ SO RAMN. – 2013. – T.33. – №6. – S.18 – 24.

Lenzen S. The mechanisms of alloxan- and streptozotocin-induced diabetes / S. Lenzen // Diabetologia. – 2008. – № 51. – Р.216 – 226.

Haklar G. Oxygen radicals and nitric oxide in rat mesenteric ischemia-reperfusion: modulation by L-arginine and NG-nitro-L-arginine methyl ester / G. Haklar, D.C. Ulukaya, M. Yuksel [et al] // Clin.Exp.Pharmacol. – 1998. – № 25(11). – Р. 908 – 912.

Dhawan V. Chronic L-arginine supplementation improves endothelial cell vasoactive functions in hepercholesterolemic and atherosclerotic monkeys / V. Dhawan, S.S. Handu, C.K. Nain [et al] // Mol.Cell Biochem. 2005. – № 269(1-2). – Р.1 – 11.

Mogilintskaia L.V. Effects of arginine on properties of the erythrocyte membranes in hypoxia / L.V. Mogilintskaia, А. Fan [et al] // Bull.Exp.Biol.Med. – 1992 – № 113 (5). – Р.457 – 498.

Vignais P.V. The superoxide-generating NADPH oxidase: structural aspects and activation mechanism / P.V. Vignais // Cell Mol.Life Sci. – 2002. – № 59(9) . – Р.1428 – 1459.

Tovmasyan M. S. Izmeneniye urovnya i aktivnosti antioksidantnykh i prooksidantnykh metalloproteinov tkaney krys pod vliyaniyem L-arginina / M. S. Tovmasyan , G.S.Khachatryan, R.M.Simonyan // Meditsinskaya nauka Armenii. – 2008. – T. 48. – № 1. – C. 35 – 46.




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