Fine needle biopsy in differential diagnosis of nodular goiter with autoimmune thyroiditis and differentiated thyroid cancers

M. I. Sheremet, L. P. Sydorchuk, V. O. Shidlovskyi, A. D. Bedenyuk, A. I. Popovych, O. V. Lazaruk


We examined 75 women with nodular NGAIT and 12 patients with differentiated thyroid cancer during 2015-2016. We have carried out an immunohistochemical study by means of monoclonal antibodies against Fas, FasL, Bcl-2, P53 and Ki67 antigens using the thyroid gland puncture material.  

Thus, compared with known methods of differential diagnosis, the proposed study allows for preoperative stage, with a high probability differentiate NGAIT of DTC, by examining extracts from tissues of the thyroid gland, which, in turn, said the volume of surgery and treatment program.

            Abbreviations: NGAIT - nodular goiter combined with autoimmune thyroiditis, TG – thyroid gland, PCE – preoperative cytological examination, DTC- differentiated thyroid cancer.


nodular goiter with autoimmune thyroiditis, fine needle biopsy, apoptosis, proliferation, thyroid gland, differentiated thyroid cancer

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Kolomecki K., Maciaszczyk P., Stepien H., Stepien T., Kuzdak K., Ulanska J. P53 concentration and soluble FasL (sFasL) serum level as indicators of apoptosis in patients with benign and malignant thyroid tumors. Bratisl Lek Listy. 2005; 106 (10): 297-300.

Tsyganenko O.S. Voroschuk R.S. Immunomorfological reaction in the thyroid tissue in patients with autoimmune thyroiditis in combination with nodular goiter. Arta Medica. Nicholas Anestiadi, Tenth Congress of the Association of Surgeons of Moldova: Chisinau. 2007 October; 4 (25):51-52.

Orlinskaya N.Y. Features histological and cytological methods using in the diagnosis of various cancer states of thyroid. Collection of materials of the All-Russian conference with international participation "Clinical morphology of thyroid cancer". [Published in Russian]. Belgorod. 2004:42–43.

Berezkina I.S., Saprina T.V., Zima A.P. et al. The problem of molecular diagnostic test value in the differential diagnosis of a thyroid gland nodule. [Published in Russian]. Clinical and experimental thyroidology. 2016;12(1):38–45.

Gulchiy M.V. Clinical peculiarities of thyroid cancer in combination with other thyroid pathologies and without. [Published in Ukrainian]. Medicines Ukraine. 2007; 111:81–85.

Kim H.S. Y.J. Choi, Yun K.S. Features of papillary thyroid microcarcinoma in the presence and absence of lymphocytic thyroiditis. Endocrine Pathology. 2010; 21(3):149–153.

Di Pasquale H.M., Palazzo J.P., Rothstein J.L. Pathologic features of Hashimoto’s associated papillary thyroid carcinoma. Hum. Pathol. 2001; 32 (1):24–30.

Bondarenko O.O., Shponka I.S., Gritsenko P.A. The use of tumor markers in the diagnosis of morphological epithelial tumors of thyroid gland. [Published in Ukrainian]. Morphology. 2010;3(2):12–16.

Bozhok Y.M., Tavokina L.V., Abramenko I.V., Belous N.I. Preparation of morphological preparations for immunohistochemical study. [Published in Ukrainian]. UA. Patent UA 23098; 1998.

Sheremet М.І., Shidlovskyy V.О., Sydorchuk L.P. Analysis of a process of peroxidation, caspase-3 and caspase-8 in patients with autoimmune thyroiditis. Journal of Education, Health and Sport. 2015; 5(11):117–125.

Sheremet М.І., Shidlovskyy V.O., Sydorchuk L.P. Assessment of proliferation and apoptosis markers in patients with autoimmune thyroiditis. Journal of Education, Health and Sport. 2016; 6 (1):179–188.

Zubeev P.S. Place fine-needle aspiration biopsy in determining the indications for surgery in nodular colloid goiter. [Published in Russian]. Remedium. Special Edition Endocrinology. 2005:93–94.

Khmelnitsky D.C. Cytological and histological diagnosis of thyroid diseases: a guide. St. Petersburg., 2002:288.

Kazakov S.Р., Кushlinsky N.Ye. The investigation of CD 95, p53, bcl-2 and Ki-67 markers in autoimmune thyroid pathology patients. First Joint Meeting of European National Societies of Immunology Under the auspices of EFIS and 16th European Congress of Immunology: ECI. 2006 Sept. 6-9; Paris, France: 547.

Ganchevska P., Murdjev K., Sarafian V. Expression of proliferative antigens in human thyroid diseases. Trakia Journal of Sciences. 2004; 2(1):16–20.

Yuan Zhou, Hong-Gang Jiang, Ning Lu at. al. Chen Expression of Ki67 in Papillary Thyroid Microcarcinoma and its Clinical Significance. Asian Pacific Journal of Cancer Prevention. 2015;16 (4):1605–1608.

Choudhury M, Singh S, Agarwal S. Diagnostic utility of Ki67 and p53 immunostaining on solitary thyroid nodule - a cytohistological and radionuclide scintigraphic study. Indian J Pathol Microbiol. 2011; 54 (3):472–475.

Chumakov P.M. The function of the p53 gene: the choice between life and death. Biochemistry. 2000; 65:34–47.

Sydorchuk LP, Amosova KM. Influence of pharmacogenetically determined treatment on parameters of peripheral hemodynamics in patients with arterial hypertension. The New Armenian Medical J. 2011; 5 (2):35-43.

Pujani M, Arora B, Pujani M, et al. Role of Ki-67 as a proliferative marker in lesions of thyroid. Indian J Cancer. 2010; 47 (3):304–307.



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