LIPID PEROXIDATION SYSTEM AND ANTIOXIDANT DEFENCE STATE IN PATIENTS WITH CHRONIC GENERALIZED PERIODONTITIS SEVERITY STAGES I-II COMBINED WITH PARASITOSES

Natal'ya Savel'eva

Abstract


The article presents the results of research on the state of lipid peroxidation and antioxidant defense in patients with chronic generalized periodontitis (CGP) severity stages I-II combined, with parasitoses.
The aim of the study was to investigate lipid peroxidation and antioxidant defense in patients with chronic generalized periodontitis (CGP) severity stages I-II with parasitoses (enterobiasis, toxocariasis, giardiasis).
The study was conducted on 540 patients with chronic generalized periodontitis severity stages I-II combined with parasitic invasion on the background (the main groups). The comparison group consisted of 90 patients with CGP severity stages I-II without a parasitic invasion. The control group consisted of 30 patients without periodontal disease and chronic diseases of other organs and systems, who were considered relatively healthy during the study period.
To evaluate the intensity of free radical oxidation a screening method of induced blood serum chemiluminescence was used. Catalase activity was determined spectrophotometrically by the decrease of hydrogen peroxide quantity in the medium and superoxide dismutase (SOD) activity was determined in the test with nitroblue tetrazolium. Statistical analysis of the materials was conducted using the methods of mathematical statistics for the analysis of the obtained data.
It was found that as the disease progresses and CGP severity stage I changes into severity stage II, lipid peroxidation processes intensify in blood serum and tissues of the body. So, there was a significant increase in the content of lipid peroxidation products: diene conjugates (DC) in groups of patients with parasitoses as compared with the control group - in CGP severity stage I DC content was higher by 30-33%, in CGP severity stage II DC content was higher by 40-41%; in CGP severity stage I triene conjugates (TC) content was higher by 22-24%, in CGP severity stage II triene conjugates (TC) content was higher by 26-27%; content of lipid peroxidation (LP) end products — polymeric fluorescent Schiff bases (SB) — in CGP severity stage I was higher by 34-36%, in CGP severity stage II was higher by 45-47%. Also, in comparison with the control group a significant decrease in the activity of superoxide dismutase (SOD) was observed in patients with parasitoses: in CGP severity stage I it decreased by 20-23%, in CGP severity stage II by 23-26%. However, in patients of the main groups catalase content reduction was recorded: in CGP severity stage I by 22-24%, in CGP severity stage II by 26-27%. It is noted that between patients with CGP severity stages I-II with parasitoses and CGP patients without parasitoses significant differences were determined only in catalase and superoxide dismutase activity, indicating a more severe antioxidant defense disturbance in patients with parasitoses.


Keywords


chronic generalized periodontitis; parasitoses; lipid peroxidation; antioxidant defense

Full Text:

PDF (Russian)

References


Список литературы:

Бодня Е. И. Клинико-иммунологические аспекты паразитарных болезней / Е. И. Бодня, И. П. Бодня // Клиническая иммунология. Аллергология. Инфектология. – 2007. – № 8. – С. 18-24.

Бодня Е. И. Роль паразитарных инвазий в развитии патологии органов пищеварения / Е. И. Бодня // Сучасна гастроентерологія. – 2006. – № 3(29). – С. 56–62.

Гарюк Г. И. Паразитозы человека: современные аспекты влияния на реактивность организма и актуальность при риносинуситах у детей / Г. И. Гарюк, Е. И. Бодня, И. В. Филатова, А. Н. Головко // Журнал вушних, носових і горлових хвороб. – 2009. – № 4. – С. 72–77.

Кашкалда Д. А. Сезонные изменения про- и антиоксидантной системы как показатели адаптационных возможностей организма подростков из семей ликвидаторов последствий аварии на ЧАЭС / Д. А. Кашкалда, Г. А. Бориско // Вісник Харківського національного університету імені В. Н. Каразіна. Серія : Біологія. – 2008. – № 828, вип. 8. – С. 5-9. – Режим доступу : http://nbuv.gov.ua/j-pdf/VKhb_2008_828_8_4.pdf

Королев В. А. Изменение прооксидантно-антиоксидантного баланса при хронической интоксикации банколом и эффективность профилактических мероприятий с применением мексидола / В. А. Королев, Ю. Д. Ляшев // Курский научно-практический вестник «Человек и его здоровье». – 2014. – № 2. – С. 19-22.

Ланкин В. З. Антиоксиданты в комплексной терапии атеросклероза: pro et contra / В. З. Ланкин, А. К. Тихазе, Ю. Н. Беленков // Кардиология. – 2004. – №2. – С. 72-81.

Зенков Н. К. Окислительный стресс. Интенсивность реакций ПОЛ ограничивается антиоксидантной системой / Н. К. Зенков, В. З. Ланкин.– М. : МАИК "Наука / Интерпериодика", 2001.– 343 с.

Барабой В. А. Окислительно-антиоксидантный гомеостаз в норме и патологи / В. А. Барабой, Д. А. Сутковой. – К. : Наукова думка, 1997. – 420 с.

Нагорная Н. В. Оксидативный стресс: влияние на организм человека, методы оценки / Н. В. Нагорная, Н. А. Четверик // Здоровье ребенка.– 2010.– № 2(23).– С. 140–145.

Halliwell B. Reactive oxygen species in living systems / B. Halliwell // Amer. Journal of Med.– 1991.– Vol. 91.– P. 14–23.

Меньщикова Е. Б. Антиоксиданты и ингибиторы радикальных окислительных процессов / Е. Б. Меньщикова, Н. К. Зенков // Успехи современной биологии.– 1993.– Т. 113, Вып. 4.– С. 442–454.

Wendel A. Enzymes acting against reactive oxygen / A. Wendel // Enzymes: Tools and Targets.– Basel: Karger, 1988.– P. 161–167.

Eaton J. W. Catalases and peroxidases and glutathione and hydrogen peroxide: Mysteries of the bestiary / J. W. Eaton // J. Lab. and Clin. Med.– 1991.– Vol. 118.– P. 3–4.

Полиморфизм генов антиоксидантных ферментов при хроническом пародонтите / В. А. Трофимов, А. П. Власов, Р. В. Адамчик [и др.] // Современные проблемы науки и образования. – 2014. – №6. – Режим доступа : http://www.science-education.ru/120-15941.

Совершенствование терапии хронического пародонтита в зависимости от патогенетических вариантов течения / Е. В. Кондюрова, Р. А. Адамчик, В. А. Трофимов, В. А. Прытков, А. П. Власов // Современные проблемы науки и образования. – 2015. – №1. – Режим доступа : http://www.science-education.ru/121-17678.

Гмурман В. Е. Теория вероятностей и математическая статистика / В. Е. Гмурман.– М. : Высшее образование, 2007. – 479 с.

Гланц С. Медико-биологическая статистика / С. Гланц. – М. : Изд-во Практика, 1999.– 459 с.

Лакин Г. Ф. Биометрия / Г. Ф. Лакин. – М. : Высшая школа, 1990. – 352 с.

References:

Bodnya E.I., Bodnya I.P. Clinical and immunological aspects of parasitic diseases. Klinicheskaya immunologiya. Allergologiya. Infektologiya. 2007;8:18-24.

Bodnya E. I. The role of parasitic invasions in the development of diseases of the digestive system. Suchasna gastroenterologija. 2006;3(29):56-62.

Garyuk G.I., Bodnya E.I., Filatova I.V., Golovko A.N. Parasitosis of human: modern aspects of the impact on the reactivity and relevance in rhinosinusitis at children. Zhurnal vushnyh, nosovyh i gorlovyh hvorob. 2009;4:72-77.

Kashkalda D.A., Borisko G.A. Seasonal changes in pro- and antioxidant system as indicators of adaptive capacity of the organism of teenagers from families of the liquidators of the Chernobyl accident. Visnyk Harkivs'kogo nacional'nogo universytetu imeni V. N. Karazina. 2008;828:5-9: access mode: http://nbuv.gov.ua/j-pdf/VKhb_2008_828_8_4.pdf.

Korolev V.A., Lyashev Yu.D. Change of prooxidant-antioxidant balance in chronic intoxication with benzene and the effectiveness of preventive activities with mexidol. Kurskiy nauchno-prakticheskiy vestnik «Chelovek i ego zdorov'e». 2014;2:19-22.

Lankin V. Z. Antioksidanty v kompleksnoj terapii ateroskleroza: pro et contra / V. Z. Lankin, A. K. Tihaze, Ju. N. Belenkov // Kardiologija. – 2004. – #2. – S. 72-81.

Zenkov N.K., Lankin V.Z. Okislitel'nyy stress. Intensivnost' reaktsiy POL ogranichivaetsya antioksidantnoy sistemoy [Oxidative stress. The intensity of the reactions of lipid peroxidation is limited by the antioxidant system]. Moskva, MAIK Nauka Interperiodika, 2001:343.

Baraboy V.A., Sutkovoy D.A. Okislitel'no-antioksidantnyy gomeostaz v norme i patologi [The oxidation-antioxidant homeostasis in normal and pathological]. Kiev, Naukova dumka, 1997:420.

Nagornaya N.V., Chetverik N.A. Oxidative stress: the impact on the human body, evaluation methods. Zdorov'e rebenka. 2010;2(23):140-145.

Halliwell B. Reactive oxygen species in living systems. Amer. Journal of Med. 1991;91:14–23.

Men'shchikova E.B., Zenkov N. K. Antioxidants and inhibitors of radical oxidation processes. Uspekhi sovremennoy biologii. 1993;113(4):442-454.

Wendel A. Enzymes acting against reactive oxygen. Enzymes: Tools and Targets. Basel, Karger, 1988:161-167.

Eaton J.W. Catalases and peroxidases and glutathione and hydrogen peroxide: Mysteries of the bestiary. J. Lab. and Clin. Med. 1991;118:3-4.

Trofimov V.A., Vlasov A.P., Adamchik R.V., Kondyurova E.V., Prytkov V.A. Polymorphism of genes of antioxidant enzymes at chronic periodontitis. Sovremennye problemy nauki i obrazovaniya. 2014;6: access mode: http://www.science-education.ru/120-15941.

Kondyurova E.V., Adamchik R.A., Trofimov V.A., Prytkov V.A., Vlasov A.P. Improving the treatment of chronic, depending on the pathogenic variants of the. Sovremennye problemy nauki i obrazovaniya. 2015;1: access mode: http://www.science-education.ru/121-17678.

Gmurman V.E. Teoriya veroyatnostey i matematicheskaya statistika [Theory of Probability and Mathematical Statistics]. Moskva, Vysshee obrazovanie, 2007:479.

Glants S. Mediko-biologicheskaya statistika [Biomedical Statistics]. Moskva, Izd-vo Praktika, 1999:459.

Lakin G. F. Biometriya [Biometrics]. Moskva, Vysshaya shkola, 1990:352.


Refbacks

  • There are currently no refbacks.




Copyright (c) 2015 Journal of Education, Health and Sport



Journal of Education, Health and Sport formerly Journal of Health Sciences

Declaration on the original version.

Editors indicates that the main version of the magazine is to issue a "electronic".

The journal has had 5 points in Ministry of Science and Higher Education parametric evaluation. § 8. 2) and § 12. 1. 2) 22.02.2019.

1223 Journal of Education, Health and Sport eISSN 2391-8306 7

ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

Archives 2011 - 2014

PBN 2011 - 2014

https://pbn.nauka.gov.pl/sedno-webapp/search?search&searchCategory=WORK&filter.inJournal=36616

POL-index 2011 - 2014

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-32a319c5-a850-4f44-ba70-1418a6087655

BASE 2011 - 2014

https://www.base-search.net/Search/Results?lookfor=Journal+of+Health+Sciences+%28Radom%29&type=all&oaboost=1&ling=1&name=&thes=&refid=dcrespl&newsearch=1

http://elibrary.ru/contents.asp?titleid=37467

http://journal.rsw.edu.pl/index.php/JHS/issue/archive

Indexed in Bases, Bazy indeksacyjne: ERIH Plus, Worldcat, PBN/POL-Index, ICI Journals Master List, Directory of Open Access Journals (DOAJ), ZBD, Ulrich's periodicals, Google Scholar, Polska Bibliografia Lekarska

US NLM = 101679844

101679844 - NLM Catalog Result - NCBI

https://www.ncbi.nlm.nih.gov/nlmcatalog/101679844

Find a library that holds this journal: http://worldcat.org/issn/23918306

Journal Language(s): English 

PBN Poland

https://pbn.nauka.gov.pl/sedno-webapp/journals/49068

POL-index

https://pbn.nauka.gov.pl/polindex-webapp/browse/journal/journal-c39c8169-88d2-45db-9e7f-d948ce9981c4

BASE

https://www.base-search.net/Search/Results?join=AND&bool0[]=AND&lookfor0[]=2391-8306&type0[]=all&page=11&l=pl&oaboost=1&refid=dcpagepl

Redaction, Publisher and Editorial Office

Instytut Kultury Fizycznej Uniwersytet Kazimierza Wielkiego w Bydgoszczy, Institute of Physical Education Kazimierz Wielki University in Bydgoszcz, Poland 85-091 Bydgoszcz ul. Sportowa 2  www.ukw.edu.pl Copyright by Instytut Kultury Fizycznej UKW w Bydgoszczy http://ojs.ukw.edu.pl/index.php/johs  Open Access ISSN 2391-8306 formerly ISSN: 1429-9623 / 2300-665X

The journal has been approved for inclusion in ERIH PLUS.

The ERIH PLUS listing of the journal is available at https://dbh.nsd.uib.no/publiseringskanaler/erihplus/periodical/info?id=485984

SIC Science citation index (calculated on the basis of TCI and Page Rank) 0

Russian Impact factor 0.16

Indexed in Index Copernicus Journals Master List.

http://journals.indexcopernicus.com/Journal+of+Education+Health+and+Sport,p24782242,3.html

ICV 2018 = 95.95 ICV 2017 = 91.30 ICV 2016 = 84.69 ICV 2015 = 93.34 ICV 2014 = 89.51 Standardized Value: 8.27 ICV 2013: 7.32 ICV 2012: 6.41 ICV 20115.48

The InfoBase Index IBI Factor for the year 2015 is 3.56 in InfoBase Index.com.

Website: www.infobaseindex.com

Universal Impact Factor 1.78 for year 2012. (http://www.uifactor.org/AppliedJournals.aspx)

Indexed in Polish Scholarly Bibliography (PBN) (PBN Polska Bibliografia Naukowa) (https://pbn.nauka.gov.pl/journals/36616)

is a portal of the Polish Ministry of Science and Higher Education, collecting information on publications of Polish scientists and on Polish and foreign scholarly journals. Polish Scholarly Bibliograhpy is a part of POL-on - System of Information on Higher Education. It is operated by the Interdisciplinary Centre for Mathematical and Computational Modelling, University of Warsaw.

Indexed in Russian Sciences Index Российский индекс научного цитирования (РИНЦ) http://elibrary.ru/contents.asp?titleid=37467

Indexed in Arianta Polish scientific and professional electronic journals Aneta Drabek i Arkadiusz Pulikowski

(http://www1.bg.us.edu.pl/bazy/czasopisma/czasop_full.asp?id=3595)